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micro bio spin p 30 tris chromatography columns  (Bio-Rad)


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    Bio-Rad micro bio spin p 30 tris chromatography columns
    Micro Bio Spin P 30 Tris Chromatography Columns, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 98/100, based on 21519 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/micro+bio+spin+p+30+tris+chromatography+columns/Tris/pmc13013651-502-5-12
    Average 98 stars, based on 21519 article reviews
    micro bio spin p 30 tris chromatography columns - by Bioz Stars, 2026-10
    98/100 stars

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    Chromatography:

    Article Title: ERCC6L2 ensures repair fidelity for staggered-end DNA double-strand breaks.
    Article Snippet: The X12-3 oligonucleotide was labelled at the 3' terminus with (ɑ-P) dCTP (Hartmann Analytic) and Terminal Transferase (New England Biolabs) according to manufacturer's instructions. .. Unincorporated nucleotides were removed with Micro Bio Spin P-30 Tris chromatography columns (Bio-Rad). .. Unincorporated nucleotides were removed with Micro Bio Spin P-30 Tris chromatography columns (Bio-Rad).

    Article Title: Mechanism of BRCA1–BARD1 function in DNA end resection and DNA protection
    Article Snippet: The resulting linear dsDNA was labelled by fill-in at the 3′ end with 0.25 mM of (α- 32 P)dCTP (Hartmann Analytic), dGTP, dATP and 5 U of the Klenow fragment of DNA polymerase I exo- (New England Biolabs). .. Unincorporated nucleotides were removed using Micro Bio-Spin P-30 Tris chromatography columns (BioRad). .. For the ATPase assay with wild-type DNA2 and helicase-dead DNA2 D277A, the 10.3 kbp-long pFB-MBP-hMLH3 plasmid was linearized with NheI (New England Biolabs) and purified with QIAquick PCR purification kit (Qiagen).

    Article Title: IFI16 senses and protects stalled replication forks.
    Article Snippet: The DNA substrates for the in vitro assays were radiolabeled at the 3ꞌ terminus with [α- 32 P]dCTP (Hartmann Analytic) and terminal transferase (New England Biolabs) according to the manufacturer’s instructions. .. Unincorporated nucleotides were removed using Micro Bio-Spin P-30 Tris chromatography columns (Biorad). .. The dialyzed protein was aliquoted, snap-frozen in liquid nitrogen and stored at -80 ◦ C. MRE11, WRN, DNA2 and RPA were expressed in Sf9 cells in SFX Insect serum-free medium (Hyclone) using the Bac-to-Bac expression system (Invitrogen), according to manufacturer’s recommendations.

    Article Title: ZNF280A links DNA double-strand break repair to human 22q11.2 distal deletion syndrome.
    Article Snippet: DNA double-strand breaks (DSB) are among the most deleterious forms of DNA damage and, if unresolved, result in DNA mutations and chromosomal aberrations that can cause disease, including cancer.. Repair of DSBs by homologous recombination requires extensive nucleolytic digestion of DNA ends in a process known as DNA-end resection.. In recent years, progress has been made in understanding how this process is initiated, but the later stages of this process—long-range DNA-end resection—are not well understood.

    Article Title: IFI16 senses and protects stalled replication forks
    Article Snippet: The DNA substrates for the in vitro assays were radiolabeled at the 3ꞌ terminus with [α- 32 P]dCTP (Hartmann Analytic) and terminal transferase (New England Biolabs) according to the manufacturer’s instructions. .. Unincorporated nucleotides were removed using Micro Bio-Spin P-30 Tris chromatography columns (Biorad). ..

    Article Title: EXO1 promotes the meiotic MLH1-MLH3 endonuclease through conserved interactions with MLH1, MSH4 and DNA
    Article Snippet: X12-3 and PC1253 were 32 P-labeled at the -3’ terminus using (α- 32 P)dCTP (Hartmann Analytic) and terminal transferase (New England Biolabs) according to the manufacturer’s instructions. .. Unincorporated (α- 32 P)dCTP was removed using the Micro Bio-Spin P-30 Tris chromatography columns (BioRad). .. The 2.2 kbp substrate was randomly labeled by amplifying the human NBS1 gene in a PCR reaction containing 66 nM (α- 32 P) dCTP (Hartmann Analytic) along with the standard concentration of dNTPs (200 μM each) .

    Article Title: ERCC6L2 ensures repair fidelity for staggered-end DNA double-strand breaks
    Article Snippet: .. Unincorporated nucleotides were removed with Micro Bio Spin P-30 Tris chromatography columns (Bio-Rad). .. Helicase assays were carried out in 15 mL of final volume in buffer containing 25 mM Tris acetate (pH 7.5), 20 mM NaCl, 2 mM magnesium acetate, 1 mM dithiothreitol, 0.1 mg/mL recombinant albumin (New England Biolabs) and 1 nM DNA substrate.

    Article Title: Nuclear mechanostability emerges from satellite DNA condensation into chromocenters
    Article Snippet: Subsequently, imidazole was added to a final concentration of 10 mM and the solution was incubated with pre-equilibrated Ni-NTA agarose resin (Qiagen) at 4°C for 1 h, in agitation. .. Free nucleotides were removed with Micro Bio-Spin P-30 Tris chromatography columns (Biorad). .. Cy-3 labelled plasmid DNA for in vitro phase separation experiments were produced from pMK-RQ_192xAATAT (pMK-RQ backbone with an inserted tract containing roughly 192 repeats of AATAT) using the Label IT ® Nucleic Acid Labeling Kit (MIR 3625, mirus bio) according to the manufacturer’s instructions.



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